Alcian Blue dye is used to prove glycosaminoglycan in mucins, for staining amyloids, cysteines and for polychromatic staining of mastocytes according to Alcian-Blue Safranin. It is also used to determine bacterial species and detecting bacterial capsules. Alcian Blue pH 1.0 kit enables adequate staining and visualisation of heavily sulfated mucopolysaccharides without staining carboxylated acid mucins or neutral mucins. Cellular nuclei are stained red using a counterstain.
Alcian Blue pH 1.0 kit
Three-reagent kit for staining heavily sulfated mucopolysaccharides. Slides are counterstained with Nuclear Fast Red (Kernechtrot) reagent to fully detect the presence of Alcian Blue positive staining.
Description
Additional information
Size | |
---|---|
Brand | |
Stain pack | |
Stain Category | Carbohydrates |
Related products
Stain Kits
Alcian Yellow Toluidine Blue kit
Six-reagent kit for staining Helicobacter pylori in gastric tissue sections. This method is one of the most popular non-silver methods for staining of H. pylori, where bacteria are stained blue in contrast to yellow mucins.
Stain Kits
TB-Stain Histo kit
Three-reagent kit for staining acid-fast bacteria (pathogenic mycobacteria) in histology sections, sputum, smears and culture smears according to Ziehl-Neelsen. Heating of the carbol-fuchsin solution is avoided in this protocol hence omitting the release of hazardous phenolic vapours.
Stain Kits
BioGram Histo kit
Five-reagent kit for identification of bacteria according to Gram. For differentiation between Gram-positive and Gram-negative bacteria in histology sections.
Stain Kits
Hematoxalyin Instant Kit
Two-component kit that contains ready-to-use powders for the preparation of hematoxylin reagent for progressive and regressive nuclear staining in histopathology and cytology.
1 Litre bottles
Stain Kits
P.A.S. Diastase Kit
BioGnost’s P.A.S. Diastase kit is most commonly used for identifying glycogen in liver. Periodic acid enables the molecules containing glycol groups to create aldehydes affected by Schiff’s reagent staining them violet (magenta). Specific stains are created by applying the PAS method on unsubsti-tuted polysaccharides, mucoproteins and glycoproteins, glycolipids and phospholipids. Alpha-amylase enzyme (also known as diastasis) is used for differentiation between glycogen and other PAS-positive structures by dissolving 1→4 glycosidic bonds, causing the glycogen to remain unstained after the PAS reaction. BioGnost’s P.A.S. Diastase kit uses thermostable enzyme which does not require heating to +37°C to be active, but incubat-ing the section at +37°C is preferred in order to achieve better glycogen breakdown. The same tissue section is used as negative control for this reaction, but the sample is not treated using alpha-amylase.
For 100 tests.
Stain Kits
Elastica-Van Gieson kit
Four-reagent kit for staining elastic fibres and differentiation between elastic tissue, collagen and other types of connective tissue. The rapid method enables a satisfactory result with shorter section staining time.
Stain Kits
TB-Stain Fluorescent Kit
Three-reagent kit for fluorescence-microscopic detection of acid-fast bacteria. Contains TB Auramine-Rhodamine reagent, double amount of TB Decolorizer Fluorescent and TB Permanganate reagent as counterstain.
4 x 100ml bottles.
Martius Scarlet Blue (MSB) kit, 6x100ml+1x250ml
Seven-reagent kit used for fibrin visualisation, especially of older clusters. This method is a modification of Masson Trichrome and is ideal for studying connective tissue and vascular pathology.