Many bacterial cells are easily stained by using simple dyes or Gram stain. However, a few strains of bacteria, such as Mycobacteria and Nocardia cannot be stained using simple dyes (the results may vary significantly if successfully stained). Cell walls of the Mycobacteria strain contain fatty acids which make them hard to stain. In order to stain the walls, a higher concentration of dye or a longer period of heating is required. However, once stained, the dye is ever more difficult to remove from the cells. Those bacteria are called acid-fast because they remain their primary colour even after being treated with acid alcohol (3% HCl alcohol solution). Fluorescence has been used to detect acid-fast bacteria for many years. This method is more sensitive than the Kinyoun method. It takes less time to interpret the results. Auramine-Rhodamine, acid alcohol (0.75% HCl alcohol solution) as a differentiation medium and potassium permanganate as a contrasting dye are used in this method.
TB-Stain Fluorescent Kit
Three-reagent kit for fluorescence-microscopic detection of acid-fast bacteria. Contains TB Auramine-Rhodamine reagent, double amount of TB Decolorizer Fluorescent and TB Permanganate reagent as counterstain.
4 x 100ml bottles.
Elastica-Van Gieson kit
Four-reagent kit for staining elastic fibres and differentiation between elastic tissue, collagen and other types of connective tissue. The rapid method enables a satisfactory result with shorter section staining time.
Warthin Starry kit
Five-reagent kit for staining Spirochaeta, Helicobacter pylori, Microsporidia and Legionella pneumophila. The kit contains 12 jars with gelatine that enables both incubation and staining of sections, as well as other reagents that enable precipitation of silver on the bacterial surface. The bacteria are found in the mucus of the surface epithelium, in the apical gastric glands and in the gastric mucosa.
Gomori Trichrome kit
Five-reagent kit for staining muscle, collagen fibre and nuclei, contains blue counterstain. The kit can be used to contrast skeletal, cardiac or smooth muscle.
HE Rapid Staining kit- frozen and paraffin sections
Ready-to-use eight-reagent kit (in 16 containers that can be used as staining jars) for rapid HE staining of frozen and paraffin tissue sections in histopathology. Contains xylene substitute as clearing agent and xylene substitute-based medium for permanent section covering.
For 100 tests.
Martius Scarlet Blue (MSB) kit, 6x100ml+1x250ml
Seven-reagent kit used for fibrin visualisation, especially of older clusters. This method is a modification of Masson Trichrome and is ideal for studying connective tissue and vascular pathology.