Von Kossa kit is used for calcium deposits and calcium salt visualisation. Silver ions from silver nitrate replace carbonate and phosphate calcium ions that, under a strong source of light, create a microscopically visible silver glow. Treating the preparation with lithium carbonate prevents false-positive staining. Counterstaining is achieved with Nuclear Fast Red (Kernechtrot) reagent.
Von Kossa kit
Five-reagent kit for simple and reproducible detection of calcium deposits and calcium salt in tissue samples according to Von Kossa. Tissue calcification is associated with metabolic problems in various tissues (like bone marrow and mamma) and in tumours.
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Stain Category | Pigments and Mineral Deposits |
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