BioGnost’s Bio-Diff RTU kit enables rapid, simple and high-quality staining according to May-Gruenwald staining method. Except for standard staining of blood smears, the kit may be used for staining parasites and fungae, histology samples embedded in paraffin, and cytology smears. Advantages of Bio-Diff RTU kit: extremely rapid staining (14 seconds) of blood and cytology smears; practical and simple for use owing to impermeable polypropylene jars filled with 100 ml of reagent that enable direct dipping of sections (for 100-200 tests); buffered solutions that enable consistent quality in staining each section. Each part of the set is stabilised separately and prepared according to the highest standards.
Bio-Diff RTU kit
Ready-to-use three-reagent kit with reagents stored in containers that can be used as staining jars. Kit contains fixative agent, red and blue components for fast and effective staining and buffer tablet for consistent staining results.
Description
PROCEDURE
Histological sections staining procedure | ||
Fix the sample (Formaldehyde NB 4%, Formaldehyde NB 10%), rinse with water and dehydrate through series of ascending alcohol solutions (Histanol 70, Histanol 80, Histanol 95 and Histanol 100). | ||
1 | Deparaffinize the section in xylene (BioClear) or in a xylene substitute (BioClear New) | 3 exchanges, 10 minutes each |
2 | Rehydrate using 100% alcohol (Histanol 100) | 2 exchanges, 5 and 3 minutes |
3 | Rehydrate using 95% alcohol (Histanol 95) | 2 minutes |
4 | Rehydrate in distilled (demi) water | 2 minutes |
5 | Dip the section into Bio-Diff 2 RTU reagent and gently stir | 7 seconds |
6 | Dip the section into Bio-Diff 3 RTU reagent and gently stir | 5 seconds |
7 | Rinse the smear using Buffer solution pH 7.2 | 1 minute (with agitation) |
8 | Decant the excessive reagent from the section onto filter paper | |
9 | Dehydrate and differentiate in 95% alcohol (Histanol 95) while gently stirring | 10 seconds |
10 | Dehydrate the section by using 100% alcohol (Histanol 100) | 1 minute |
11 | Clear the section in xylene (BioClear) or in a xylene substitute (BioClear New) | 2 exchanges, 5 minutes each |
Cytobacteriology samples staining procedure (urine, punctates, CSF) | ||
1 | Let the cytology smear dry | |
2 | Dip the section into Bio-Diff 1 RTU reagent | 5 seconds |
Note: Incubate CSF for a longer period of time | 1 minute | |
3 | Decant the excessive reagent from the section onto filter paper | |
4 | Dip the section into Bio-Diff 2 RTU reagent | 3 x 1 second (CSF 2 x 1 second) |
Note: extend the incubation period if a stronger hue of red/purple is required | Do 5 x 1 second | |
5 | Decant the excessive reagent from the section onto filter paper | |
6 | Dip the section into Bio-Diff 3 RTU reagent | 6 x 1 seconds (CSF 2 x 1 second) |
Note: decrease the incubation period if a stronger hue of red/purple is required | 5 x 1 second | |
7 | Rinse the section in pH 7.2 buffer solution | 1 minute (with agitation) |
8 | Dry the preparation | |
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