Bio-Diff RTU kit

Fix the sample (Formaldehyde NB 4%, Formaldehyde NB 10%), rinse with water and dehydrate through series of ascending alcohol solutions (Histanol 70, Histanol 80, Histanol 95 and Histanol 100).
Clear the sample with intermedium; in xylene (BioClear) or in a xylene substitute (BioClear New).
Infiltrate and fit the sample in paraffin (BioWax Plus 56/58, BioWax 56/58, BioWax Blue, BioWax Micro).
Cut the paraffin block to 4-6 μm slices and mount them on a VitroGnost Super Grade glass slide.

1

Deparaffinize the section in xylene (BioClear) or in a xylene substitute (BioClear New)

2

Rehydrate using 100% alcohol (Histanol 100)

2 exchanges, 5 and 3 minutes

3

Rehydrate using 95% alcohol (Histanol 95)

Rehydrate in distilled (demi) water

5

Dip the section into Bio-Diff 2 RTU reagent and gently stir

Dip the section into Bio-Diff 3 RTU reagent and gently stir

Rinse the smear using Buffer solution pH 7.2

8

Decant the excessive reagent from the section onto filter paper

9

Dehydrate and differentiate in 95% alcohol (Histanol 95) while gently stirring

10 seconds

Dehydrate the section by using 100% alcohol (Histanol 100)

11

Clear the section in xylene (BioClear) or in a xylene substitute (BioClear New)

2 exchanges, 5 minutes each

1

Let the cytology smear dry

2

Dip the section into Bio-Diff 1 RTU reagent

Note: Incubate CSF for a longer period of time

Decant the excessive reagent from the section onto filter paper

Dip the section into Bio-Diff 2 RTU reagent

Note: extend the incubation period if a stronger hue of red/purple is required

5

Decant the excessive reagent from the section onto filter paper

6

Dip the section into Bio-Diff 3 RTU reagent

6 x 1 seconds (CSF 2 x 1 second)

Note: decrease the incubation period if a stronger hue of red/purple is required

5 x 1 second 

7

Rinse the section in pH 7.2 buffer solution

1 minute (with agitation)

8

Dry the preparation