Many bacterial cells are easily stained by using simple dyes or Gram stain. However, a few strains of bacteria, such as Mycobacteria and Nocardia cannot be stained using simple dyes (the results may vary significantly if successfully stained). Cell walls of the Mycobacteria strain contain fatty acids which make them hard to stain. In order to stain the cell wall, a higher concentration of dye or a longer period of heating is required. However, once stained, the dye is ever more difficult to remove from the cells. Those bacteria are called acid-fast because they retain their primary color even after being treated with acid alcohol (3% HCl alcohol solution). Fluorescence has been used to detect acid-fast bacteria for many years. This method is more sensitive than the Kinyoun method. It takes less time to interpret the results. Auramine O, Acid alcohol as a differentiation medium and potassium permanganate as a counterstain are used in this method.
TB-Stain Auramine O Kit
Three-reagent kit for staining acid-fast bacteria using fluorescence method. Contains TB Auramine O reagent, double amount of TB Decolorizer Fluorescent and counterstain of TB Permanganate reagent.
4 x 100ml bottles.
Mallory Trichrome kit
Three-reagent staining kit for connective tissue visualisation and detection of collagen, cartilage, muscle, elastic fibres, mucous, pituarity cells, reticulum, bones, amyloid and erythrocytes.
Alcian Yellow Toluidine Blue kit
Six-reagent kit for staining Helicobacter pylori in gastric tissue sections. This method is one of the most popular non-silver methods for staining of H. pylori, where bacteria are stained blue in contrast to yellow mucins.
Warthin Starry kit
Five-reagent kit for staining Spirochaeta, Helicobacter pylori, Microsporidia and Legionella pneumophila. The kit contains 12 jars with gelatine that enables both incubation and staining of sections, as well as other reagents that enable precipitation of silver on the bacterial surface. The bacteria are found in the mucus of the surface epithelium, in the apical gastric glands and in the gastric mucosa.
Elastica-Van Gieson kit
Four-reagent kit for staining elastic fibres and differentiation between elastic tissue, collagen and other types of connective tissue. The rapid method enables a satisfactory result with shorter section staining time.
Martius Scarlet Blue (MSB) kit
Seven-reagent kit used for fibrin visualisation, especially of older clusters. This method is a modification of Masson Trichrome and is ideal for studying connective tissue and vascular pathology.