Many bacterial cells are easily stained by using simple dyes or Gram stain. However, a few strains of bacteria, such as Mycobacteria and Nocardia cannot be stained using simple dyes (the results may vary significantly if successfully stained). Cell walls of the Mycobacteria strain contain fatty acids which make them hard to stain. In order to stain the cell wall, a higher concentration of dye or a longer period of heating is required. However, once stained, the dye is ever more difficult to remove from the cells. Those bacteria are called acid-fast because they retain their primary color even after being treated with acid alcohol (3% HCl alcohol solution). Fluorescence has been used to detect acid-fast bacteria for many years. This method is more sensitive than the Kinyoun method. It takes less time to interpret the results. Auramine O, Acid alcohol as a differentiation medium and potassium permanganate as a counterstain are used in this method.
TB-Stain Auramine O Kit
Three-reagent kit for staining acid-fast bacteria using fluorescence method. Contains TB Auramine O reagent, double amount of TB Decolorizer Fluorescent and counterstain of TB Permanganate reagent.
4 x 100ml bottles.
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Stains Reagents And Dyes
Stain Kits
P.A.S. Diastase Kit
BioGnost’s P.A.S. Diastase kit is most commonly used for identifying glycogen in liver. Periodic acid enables the molecules containing glycol groups to create aldehydes affected by Schiff’s reagent staining them violet (magenta). Specific stains are created by applying the PAS method on unsubsti-tuted polysaccharides, mucoproteins and glycoproteins, glycolipids and phospholipids. Alpha-amylase enzyme (also known as diastasis) is used for differentiation between glycogen and other PAS-positive structures by dissolving 1→4 glycosidic bonds, causing the glycogen to remain unstained after the PAS reaction. BioGnost’s P.A.S. Diastase kit uses thermostable enzyme which does not require heating to +37°C to be active, but incubat-ing the section at +37°C is preferred in order to achieve better glycogen breakdown. The same tissue section is used as negative control for this reaction, but the sample is not treated using alpha-amylase.
For 100 tests.
Papanicolaou Rapid Staining Kit, for 100 Tests
Ready-to-use eight-reagent kit (in 18 containers that can be used as staining jars) for rapid progressive gynecology and non-gynecology cytological samples. Contains xylene substitute as clearing agent and xylene substitute-based medium for permanent covering of samples.
PAP-100T for 100 tests
Stain Kits
BioGram Histo kit
Five-reagent kit for identification of bacteria according to Gram. For differentiation between Gram-positive and Gram-negative bacteria in histology sections.
Stain Kits
Alcian Yellow Toluidine Blue kit
Six-reagent kit for staining Helicobacter pylori in gastric tissue sections. This method is one of the most popular non-silver methods for staining of H. pylori, where bacteria are stained blue in contrast to yellow mucins.
Stain Kits
TB-Stain Cold Kit
Three-reagent kit for staining acid-fast bacteria according to Kinyoun. Contains TB Carbol Fuchsin reagent, double amount of TB Decolorizer and TB Malachite Green reagent as counterstain.
4 x 100ml bottles.
Stain Kits
Eosin-Nigrosin Vital
Fast detection (one-step detection) of sperm vitality and visualisation of dead and living sperm cells with one reagent. A simple, easy and fast method for semen analysis.
Stain Kits
Gomori Trichrome kit
Five-reagent kit for staining muscle, collagen fibre and nuclei, contains blue counterstain. The kit can be used to contrast skeletal, cardiac or smooth muscle.