Gram staining is a method of differentiating bacterial species and it is commonly known and used in microbiology. It is also one of the most frequently used diagnostic methods in hospital and clinical laboratories. Gram staining differentiates bacteria into two groups: Gram-positive and Gram-negative. That division is based on the two groups’ bacterial membrane structural differences, i.e. their capability of retaining the dye. Gram-positive bacteria have a thicker cellular membrane which enables retaining the dye inside the cell by treating them with iodine solution that creates insoluble iodine and primary dye complex. Gram-negative bacteria have thinner cellular membrane structure which cannot retain the dye. It washes away through the membrane, and using counterstaining forms the basis for differentiating between the two bacteria groups. BioGnost’s BioGram Histo kit contains Gram Crystal Violet 1% solution, stabilised Gram Lugol solution, two packages of Gram Decolouriser 2 solution, Gram Safranin solution and two packages of picric acid in acetone. Its characteristics make it an optimal bacteria staining agent which provides consistent results.
Gomori Trichrome kit
Five-reagent kit for staining muscle, collagen fibre and nuclei, contains blue counterstain. The kit can be used to contrast skeletal, cardiac or smooth muscle.
Alcian Yellow Toluidine Blue kit
Six-reagent kit for staining Helicobacter pylori in gastric tissue sections. This method is one of the most popular non-silver methods for staining of H. pylori, where bacteria are stained blue in contrast to yellow mucins.
Warthin Starry kit
Five-reagent kit for staining Spirochaeta, Helicobacter pylori, Microsporidia and Legionella pneumophila. The kit contains 12 jars with gelatine that enables both incubation and staining of sections, as well as other reagents that enable precipitation of silver on the bacterial surface. The bacteria are found in the mucus of the surface epithelium, in the apical gastric glands and in the gastric mucosa.
Elastica-Van Gieson kit
Four-reagent kit for staining elastic fibres and differentiation between elastic tissue, collagen and other types of connective tissue. The rapid method enables a satisfactory result with shorter section staining time.
TB-Stain Auramine O Kit
Three-reagent kit for staining acid-fast bacteria using fluorescence method. Contains TB Auramine O reagent, double amount of TB Decolorizer Fluorescent and counterstain of TB Permanganate reagent.
4 x 100ml bottles.
Grocott kit, stabilised
Seven-reagent kit for visualization of fungi and histological argentaffin structures in general (such as basal membranes). Green counterstain provides clear and visually rich contrast to target structures stained black.
Martius Scarlet Blue (MSB) kit, 6x100ml+1x250ml
Seven-reagent kit used for fibrin visualisation, especially of older clusters. This method is a modification of Masson Trichrome and is ideal for studying connective tissue and vascular pathology.