Many bacterial cells are easily stained by using simple dyes or Gram stain. However, a few bacterial strains, such as Mycobacteria and Nocardia cannot be stained using simple dyes (or, if successfully stained, the results may vary significantly). Cellular wall of the Mycobacteria strain contains a waxy substance – mycolic acid. Those are beta-hydroxy carboxylic acids with chains containing up to 90 carbon atoms. Its resistance to acidity is associated with mycolic acid chain length. In order to stain such strains, a higher concentration of dye or a longer period of heating is required. However, once stained, the dye is even more difficult to remove from the cells. Those bacteria are called acid-resistant because they maintain their primary colour even after decolourisation using acid alcohol (Carbol Fuchsin). Early laboratory diagnosis of tuberculosis is based on the interpretation of stained smears, and one of the best diagnostic methods is analysing sputum samples under a microscope. The most common and renowned method used for detecting the tuberculosis bacteria is staining according to Ziehl-Neelsen. This method uses Carbol Fuchsin as the main dye, acid alcohol as decolourisation medium and Methylene Blue solution as a contrasting dye. BioGnost’s TB-Stain Hot kit contains TB Carbol Fuchsin reagent, two packages of TB Decolouriser and Methylene Blue Loeffler reagent.
TB-Stain Histo kit
Three-reagent kit for staining acid-fast bacteria (pathogenic mycobacteria) in histology sections, sputum, smears and culture smears according to Ziehl-Neelsen. Heating of the carbol-fuchsin solution is avoided in this protocol hence omitting the release of hazardous phenolic vapours.
TB-Stain Fluorescent Kit
Three-reagent kit for fluorescence-microscopic detection of acid-fast bacteria. Contains TB Auramine-Rhodamine reagent, double amount of TB Decolorizer Fluorescent and TB Permanganate reagent as counterstain.
4 x 100ml bottles.
BioGram ECO kit
Four-reagent phenol-free kit for the identification of bacteria according to Gram. Kit contains Gram Crystal violet, phenol free reagent, Gram Sodium hydrogencarbon, solution, stabilized Gram Lugol solution, double amount of Gram Decolorizer solution 2 and Gram Safranin solution as counterstain.
2×50 mL+4×100 mL bottles
Alcian Yellow Toluidine Blue kit
Six-reagent kit for staining Helicobacter pylori in gastric tissue sections. This method is one of the most popular non-silver methods for staining of H. pylori, where bacteria are stained blue in contrast to yellow mucins.
HE Rapid Staining kit- frozen and paraffin sections
Ready-to-use eight-reagent kit (in 16 containers that can be used as staining jars) for rapid HE staining of frozen and paraffin tissue sections in histopathology. Contains xylene substitute as clearing agent and xylene substitute-based medium for permanent section covering.
For 100 tests.
Field kit 500ml
Ready to use two-reagent kit for rapid and efficient staining and detection of parasites in haematology samples. Primarily used for staining thin and thick blood smears (dense drop) for purpose of diagnosing blood parasites. Reagents are stored in containers that can be used as staining jars.
Martius Scarlet Blue (MSB) kit
Seven-reagent kit used for fibrin visualisation, especially of older clusters. This method is a modification of Masson Trichrome and is ideal for studying connective tissue and vascular pathology.
TB-Stain Auramine O Kit
Three-reagent kit for staining acid-fast bacteria using fluorescence method. Contains TB Auramine O reagent, double amount of TB Decolorizer Fluorescent and counterstain of TB Permanganate reagent.
4 x 100ml bottles.