Reticulin kit is used for identification and easier visualisation of argentaffin reticular fibres in connective tissue. Reticulin provides structural support. It is found in the liver, spleen and kidneys. Reticulin fibres are clearly defined in the healthy liver; necrotic and cirrhotic liver has discontinuous fibres. The visualisation is based on silver depositions on reticulin fibres. The tissue sample must be oxidized with potassium permanganate. Silver is formed from ammonia solution containing silver nitrate and is deposited in the form of brown sediment on reticulin fibres. Formalin acts as a reducing agent and accelerates the procedure. Unbound silver is washed away using sodium thiosulfate.
Reticulin kit
Seven-reagent kit for detecting argyrophilic reticulin fibres. It clearly differentiates between collagen and reticulin and nerve fibres and connective tissue. The main function of reticular fibres is to provide support and are normally found in liver, lymph nodes, spleen and kidneys.
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Additional information
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Stain Category | Muscle and Connective Tissue |
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