Gram staining is a method of differentiating bacterial species and it is commonly known and used in microbiology. It is also one of the most frequently used diagnostic methods in hospital and clinical laboratories. Gram staining differentiates bacteria into two groups: Gram-positive and Gram-negative. That division is based on the two groups’ bacterial membrane structural differences, i. e. their capability of retaining the dye. Gram-positive bacteria have a thicker cellular membrane which enables retaining the dye inside the cell by treating them with iodine solution that creates insoluble iodine and primary dye complex. Gram-negative bacteria have a thinner cellular membrane structure that cannot retain the dye. It washes away through the membrane and using counterstaining forms the basis for differentiating between the two bacteria groups. BioGnost’s BioGram 4 kit contains Gram Crystal Violet 1% solution, stabilized Gram Lugol solution, two packages of Gram Decolorizer solution 2 and Gram Safranin solution. Its characteristics make it an optimal bacteria staining agent which provides consistent results.
BioGram 4 kit
Four-reagent kit for identification of bacteria according to Gram. Kit contains Gram Crystal Violet 1% solution, stabilized Gram Lugol solution, double amount of Gram Decolorizer solution 2 and Gram Safranin solution as counterstain.
5×100 ml bottles
Related products
Martius Scarlet Blue (MSB) kit, 6x100ml+1x250ml
Seven-reagent kit used for fibrin visualisation, especially of older clusters. This method is a modification of Masson Trichrome and is ideal for studying connective tissue and vascular pathology.
Stain Kits
Elastica-Van Gieson kit
Four-reagent kit for staining elastic fibres and differentiation between elastic tissue, collagen and other types of connective tissue. The rapid method enables a satisfactory result with shorter section staining time.
Stain Kits
Warthin Starry kit
Five-reagent kit for staining Spirochaeta, Helicobacter pylori, Microsporidia and Legionella pneumophila. The kit contains 12 jars with gelatine that enables both incubation and staining of sections, as well as other reagents that enable precipitation of silver on the bacterial surface. The bacteria are found in the mucus of the surface epithelium, in the apical gastric glands and in the gastric mucosa.
Stain Kits
Alcian Yellow Toluidine Blue kit
Six-reagent kit for staining Helicobacter pylori in gastric tissue sections. This method is one of the most popular non-silver methods for staining of H. pylori, where bacteria are stained blue in contrast to yellow mucins.
Stain Kits
TB-Stain Fluorescent Kit
Three-reagent kit for fluorescence-microscopic detection of acid-fast bacteria. Contains TB Auramine-Rhodamine reagent, double amount of TB Decolorizer Fluorescent and TB Permanganate reagent as counterstain.
4 x 100ml bottles.
Stain Kits
Bio-Diff Kit 3 X 1L
Three-reagent kit that contains fixative agent, red and blue components for fast and effective staining. Each kit contains buffer tablets for consistent staining results.
3×100 ml bottles
Stain Kits
P.A.S. Diastase Kit
BioGnost’s P.A.S. Diastase kit is most commonly used for identifying glycogen in liver. Periodic acid enables the molecules containing glycol groups to create aldehydes affected by Schiff’s reagent staining them violet (magenta). Specific stains are created by applying the PAS method on unsubsti-tuted polysaccharides, mucoproteins and glycoproteins, glycolipids and phospholipids. Alpha-amylase enzyme (also known as diastasis) is used for differentiation between glycogen and other PAS-positive structures by dissolving 1→4 glycosidic bonds, causing the glycogen to remain unstained after the PAS reaction. BioGnost’s P.A.S. Diastase kit uses thermostable enzyme which does not require heating to +37°C to be active, but incubat-ing the section at +37°C is preferred in order to achieve better glycogen breakdown. The same tissue section is used as negative control for this reaction, but the sample is not treated using alpha-amylase.
For 100 tests.
Stain Kits
H.B.F.P. kit
Three-reagent Hematoxylin-Basic Fuchsin-Picric acid staining kit for detection of cardiac muscle changes after ischemia or myocardial infarction. H.B.F.P. kit is a non-enzymatic histochemical technique for detection of early myocardial ischemia with vivid contrast.