Reticulin Contrast kit is used for identification and easier visualisation of argentaffin reticular fibres in connective tissue. Reticulin provides structural support. It is found in the liver, spleen and kidneys. Reticulin fibres are clearly defined in the healthy liver; necrotic and cirrhotic liver has discontinuous fibres. The visualisation is based on silver depositions on reticulin fibres. The tissue sample must be oxidized with potassium permanganate. Silver is formed from ammonia solution containing silver nitrate and is deposited in the form of brown sediment on reticulin fibres. Formalin acts as a reducing agent and accelerates the procedure. Unbound silver is washed away using sodium thiosulfate. Reticulin Contrast kit also contains a gold chloride solution that stabilises and tones the section’s image. The kit contains Nuclear Fast Red (Kernechtrot) counterstain.
Reticulin Contrast kit
Nine-reagent kit for detecting argyrophilic reticulin fibres according to Gordon and Sweets. The kit contains gold chloride solution that enhances visualisation of reticulin fibres and it also contains Nuclear Fast Red (Kernechtrot) reagent that enables fine contrasting background.
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Additional information
| Size | |
|---|---|
| Brand | |
| Stain pack | |
| Stain Category | Muscle and Connective Tissue |
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