BioGnost’s Eosin and Nigrosin Vital is used for the detection of sperm vitality. Using the reagent is very fast (one step) and simple. During sample staining eosin Y dye enters dead sperm cells (cells with damaged plasma membrane) and stains them red. Nigrosin dye provides dark contrast for better visualisation of living, non-stained sperm cells.
Fast detection (one-step detection) of sperm vitality and visualisation of dead and living sperm cells with one reagent. A simple, easy and fast method for semen analysis.
Shake Eosin-nigrosin vital reagent bottle well
Mix equal volumes of Eosin-nigrosin vital reagent and ejaculate sample (for example, mix 50 µL of Eosin and nigrosine reagent with
Let it set for 30 seconds at room temperature
Prepare a glass slide that will contain a thin smear of the stained sample
Smear the sample on the side
Note: smear the sample according to the standardized method used in the laboratory or according to the following instructions:
Dry the sections
Note: if you wish to keep the sections, apply a suitable BioMount medium on the sample (such as BioMount DPX) and cover it with a glass slide
Study the sample under a microscopical magnification factor of 40x or under an immersion lens (100x magnification factor)
Note: image under immersion lens 100x magnification will very clearly show the difference between stained and non-stained sperm cells
Count 200 sperm cells; non-stained are classified as living sperm cells, while those stained red or pink are classified as dead
TB-Stain Quick Kit
Three-reagent kit for rapid staining of acid-fast bacteria using Kinyoun-Gabbett method. Contains TB Carbol Fuchsin reagent and TB Armand reagent as counterstain.
3 x 100ml bottles.
Gomori Trichrome kit
Five-reagent kit for staining muscle, collagen fibre and nuclei, contains blue counterstain. The kit can be used to contrast skeletal, cardiac or smooth muscle.
TB-Stain Fluorescent Kit
Three-reagent kit for fluorescence-microscopic detection of acid-fast bacteria. Contains TB Auramine-Rhodamine reagent, double amount of TB Decolorizer Fluorescent and TB Permanganate reagent as counterstain.
4 x 100ml bottles.
Warthin Starry kit
Five-reagent kit for staining Spirochaeta, Helicobacter pylori, Microsporidia and Legionella pneumophila. The kit contains 12 jars with gelatine that enables both incubation and staining of sections, as well as other reagents that enable precipitation of silver on the bacterial surface. The bacteria are found in the mucus of the surface epithelium, in the apical gastric glands and in the gastric mucosa.
Ten-reagent kit for staining collagen, elastic and muscle fibres, mucin and fibrin in tissue section. Movat kit is particularly useful when examining heart and vascular diseases.
Mallory Trichrome kit
Three-reagent staining kit for connective tissue visualisation and detection of collagen, cartilage, muscle, elastic fibres, mucous, pituarity cells, reticulum, bones, amyloid and erythrocytes.
BioGram ECO kit
Four-reagent phenol-free kit for the identification of bacteria according to Gram. Kit contains Gram Crystal violet, phenol free reagent, Gram Sodium hydrogencarbon, solution, stabilized Gram Lugol solution, double amount of Gram Decolorizer solution 2 and Gram Safranin solution as counterstain.
2×50 mL+4×100 mL bottles
Stains Reagents And Dyes