BioGnost’s Eosin and Nigrosin Vital is used for the detection of sperm vitality. Using the reagent is very fast (one step) and simple. During sample staining eosin Y dye enters dead sperm cells (cells with damaged plasma membrane) and stains them red. Nigrosin dye provides dark contrast for better visualisation of living, non-stained sperm cells.
Eosin-Nigrosin Vital
Fast detection (one-step detection) of sperm vitality and visualisation of dead and living sperm cells with one reagent. A simple, easy and fast method for semen analysis.
Description
PROCEDURE
100ml | |
1 | Shake Eosin-nigrosin vital reagent bottle well |
2 | Mix equal volumes of Eosin-nigrosin vital reagent and ejaculate sample (for example, mix 50 µL of Eosin and nigrosine reagent with |
3 | Mix well |
4 | Let it set for 30 seconds at room temperature |
5 | Prepare a glass slide that will contain a thin smear of the stained sample |
6 | Smear the sample on the side |
Note: smear the sample according to the standardized method used in the laboratory or according to the following instructions:
| |
7 | Dry the sections |
Note: if you wish to keep the sections, apply a suitable BioMount medium on the sample (such as BioMount DPX) and cover it with a glass slide | |
8 | Study the sample under a microscopical magnification factor of 40x or under an immersion lens (100x magnification factor) |
Note: image under immersion lens 100x magnification will very clearly show the difference between stained and non-stained sperm cells | |
9 | Count 200 sperm cells; non-stained are classified as living sperm cells, while those stained red or pink are classified as dead |
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