BioGnost’s Eosin and Nigrosin Vital is used for the detection of sperm vitality. Using the reagent is very fast (one step) and simple. During sample staining eosin Y dye enters dead sperm cells (cells with damaged plasma membrane) and stains them red. Nigrosin dye provides dark contrast for better visualisation of living, non-stained sperm cells.
Eosin-Nigrosin Vital
Fast detection (one-step detection) of sperm vitality and visualisation of dead and living sperm cells with one reagent. A simple, easy and fast method for semen analysis.
Description
Downloads
Related products
Stain Kits
TB-Stain Auramine O Kit
Three-reagent kit for staining acid-fast bacteria using fluorescence method. Contains TB Auramine O reagent, double amount of TB Decolorizer Fluorescent and counterstain of TB Permanganate reagent.
4 x 100ml bottles.
Stain Kits
Giemsa HP kit
Four-reagent kit for staining Helicobacter pylori in gastroscopic sections according to Lennart. Advantages of this method for detecting H. pylori are sensitive and reproducible results and easy performance.
Stain Kits
P.A.S. Diastase Kit
BioGnost’s P.A.S. Diastase kit is most commonly used for identifying glycogen in liver. Periodic acid enables the molecules containing glycol groups to create aldehydes affected by Schiff’s reagent staining them violet (magenta). Specific stains are created by applying the PAS method on unsubsti-tuted polysaccharides, mucoproteins and glycoproteins, glycolipids and phospholipids. Alpha-amylase enzyme (also known as diastasis) is used for differentiation between glycogen and other PAS-positive structures by dissolving 1→4 glycosidic bonds, causing the glycogen to remain unstained after the PAS reaction. BioGnost’s P.A.S. Diastase kit uses thermostable enzyme which does not require heating to +37°C to be active, but incubat-ing the section at +37°C is preferred in order to achieve better glycogen breakdown. The same tissue section is used as negative control for this reaction, but the sample is not treated using alpha-amylase.
For 100 tests.
Stain Kits
Gomori Trichrome kit
Five-reagent kit for staining muscle, collagen fibre and nuclei, contains blue counterstain. The kit can be used to contrast skeletal, cardiac or smooth muscle.
Stain Kits
TB-Stain Histo kit
Three-reagent kit for staining acid-fast bacteria (pathogenic mycobacteria) in histology sections, sputum, smears and culture smears according to Ziehl-Neelsen. Heating of the carbol-fuchsin solution is avoided in this protocol hence omitting the release of hazardous phenolic vapours.
Stain Kits
TB-Stain ECO Kit
Three-reagent phenol-free kit for staining acid-fast bacteria. Contains TB-Fuchsin reagent, double amount of TB Decolorizer and Methylene Blue Loeffler’s reagent as counterstain.
5 x 100ml bottles.
Stain Kits
Mallory Trichrome kit
Three-reagent staining kit for connective tissue visualisation and detection of collagen, cartilage, muscle, elastic fibres, mucous, pituarity cells, reticulum, bones, amyloid and erythrocytes.