Many bacterial cells are easily stained by using simple dyes or Gram stain. However, a few bacterial strains, such as Mycobacteria and Nocardia cannot be stained using simple dyes (or, if successfully stained, the results may vary significantly). Cellular wall of the Mycobacteria strain contains waxy substance – mycolic acid. Those are beta-hydroxy carboxylic acids with chains containing up to 90 carbon atoms. Its resistance to acidity is associated with mycolic acid chain length. In order to stain such strains, a higher concentration of dye or a longer period of heating is required. However, once stained, the dye is even more difficult to remove from the cells. Those bacteria are called acid-fast because they maintain their primary colour even after decolourisation using acid alcohol (TB Fuchsin reagent, phenol-free). Early laboratory diagnosis of tuberculosis is based on the interpretation of stained smears, and one of the best diagnostic methods is analyzing sputum sample under a microscope. The most common and renowned method used for detecting tuberculosis bacteria is staining according to Ziehl-Neelsen. This kit uses modified Ziehl-Neelsen method that contains TB Fuchsin reagent, phenol-free, acid alcohol as decolorizing agent (two packages of TB Decolorizer) and Methylene Blue solution as counterstain (Methylene Blue Loeffler reagent).
TB-Stain ECO Kit
Three-reagent phenol-free kit for staining acid-fast bacteria. Contains TB-Fuchsin reagent, double amount of TB Decolorizer and Methylene Blue Loeffler’s reagent as counterstain.
5 x 100ml bottles.
Description
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P.A.S. Diastase Kit
BioGnost’s P.A.S. Diastase kit is most commonly used for identifying glycogen in liver. Periodic acid enables the molecules containing glycol groups to create aldehydes affected by Schiff’s reagent staining them violet (magenta). Specific stains are created by applying the PAS method on unsubsti-tuted polysaccharides, mucoproteins and glycoproteins, glycolipids and phospholipids. Alpha-amylase enzyme (also known as diastasis) is used for differentiation between glycogen and other PAS-positive structures by dissolving 1→4 glycosidic bonds, causing the glycogen to remain unstained after the PAS reaction. BioGnost’s P.A.S. Diastase kit uses thermostable enzyme which does not require heating to +37°C to be active, but incubat-ing the section at +37°C is preferred in order to achieve better glycogen breakdown. The same tissue section is used as negative control for this reaction, but the sample is not treated using alpha-amylase.
For 100 tests.
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Three-reagent kit for rapid staining of acid-fast bacteria using Kinyoun-Gabbett method. Contains TB Carbol Fuchsin reagent and TB Armand reagent as counterstain.
3 x 100ml bottles.
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1 Litre bottles
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Movat kit
Ten-reagent kit for staining collagen, elastic and muscle fibres, mucin and fibrin in tissue section. Movat kit is particularly useful when examining heart and vascular diseases.
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TB-Stain Hot Kit
Three-reagent kit for staining acid-fast bacteria. Contains TB Carbol Fuchsin reagent, double amount of TB Decolorizer and Methylene Blue Loeffler’s reagent as counterstain.
4 x 100ml bottles.