Many bacterial cells are easily stained by using simple dyes or Gram stain. However, a few bacterial strains, such as Mycobacteria and Nocardia cannot be stained using simple dyes (or, if successfully stained, the results may vary significantly). Cellular wall of the Mycobacteria strain contains waxy substance – mycolic acid. Those are beta-hydroxy carboxylic acids with chains containing up to 90 carbon atoms. Its resistance to acidity is associated with mycolic acid chain length. In order to stain such strains, a higher concentration of dye or a longer period of heating is required. However, once stained, the dye is even more difficult to remove from the cells. Those bacteria are called acid-fast because they maintain their primary colour even after decolourisation using acid alcohol (TB Fuchsin reagent, phenol-free). Early laboratory diagnosis of tuberculosis is based on the interpretation of stained smears, and one of the best diagnostic methods is analyzing sputum sample under a microscope. The most common and renowned method used for detecting tuberculosis bacteria is staining according to Ziehl-Neelsen. This kit uses modified Ziehl-Neelsen method that contains TB Fuchsin reagent, phenol-free, acid alcohol as decolorizing agent (two packages of TB Decolorizer) and Methylene Blue solution as counterstain (Methylene Blue Loeffler reagent).
TB-Stain ECO Kit
Three-reagent phenol-free kit for staining acid-fast bacteria. Contains TB-Fuchsin reagent, double amount of TB Decolorizer and Methylene Blue Loeffler’s reagent as counterstain.
5 x 100ml bottles.
Field kit 500ml
Ready to use two-reagent kit for rapid and efficient staining and detection of parasites in haematology samples. Primarily used for staining thin and thick blood smears (dense drop) for purpose of diagnosing blood parasites. Reagents are stored in containers that can be used as staining jars.
Three-reagent Hematoxylin-Basic Fuchsin-Picric acid staining kit for detection of cardiac muscle changes after ischemia or myocardial infarction. H.B.F.P. kit is a non-enzymatic histochemical technique for detection of early myocardial ischemia with vivid contrast.
Gomori Trichrome kit
Five-reagent kit for staining muscle, collagen fibre and nuclei, contains blue counterstain. The kit can be used to contrast skeletal, cardiac or smooth muscle.
Elastica-Van Gieson kit
Four-reagent kit for staining elastic fibres and differentiation between elastic tissue, collagen and other types of connective tissue. The rapid method enables a satisfactory result with shorter section staining time.