Alcian Blue dye is used to prove glycosaminoglycan in mucins, for staining amyloids, cysteines and for polychromatic staining of mastocytes according to Alcian-Blue Safranin. It is also used to determine bacterial species and detecting bacterial capsules. Alcian Blue pH 2.5 kit enables adequate staining and visualisation of acid mucins without staining sulfated mucins. Rinsing solutions are of the same pH value, making the specificity of the reaction more intense. Cellular nuclei are stained red using a counterstain.
Alcian Blue pH 2.5 kit
Three-reagent kit for staining acid mucopolysaccharides according to Dorling and sulphated and carboxylated sialomucins blue. Slides are counterstained with Nuclear Fast Red (Kernechtrot) reagent to fully detect the presence of Alcian Blue positive staining.
Using kit for 100 tests
Deparaffinize the section in xylene (BioClear) or in a xylene substitute (BioClear New)
3 exchanges, 2 minutes each
Rehydrate using 100% alcohol (Histanol 100)
2 exchanges, 5 and 3 minutes
Rehydrate using 95% alcohol (Histanol 95)
Rehydrate in distilled (demi) water
Stain using Alcian Blue solution pH 2.5 (add ≥5 drops)
Tilt the section and remove Alcian Blue solution pH 2.5. Without rinsing, cover the section with Sodium tetraborate, solution (add ≥5 drops)
Rinse in distilled (demi) water
Stain with Nuclear Fast Red (Kernechtrot) reagent (add ≥5 drops)
Rinse in distilled (demi) water
Dehydration using 70% alcohol (Histanol 70)
Dehydrate using 95% alcohol (Histanol 95)
Dehydrate using 100% alcohol (Histanol 100)
Clear the section in xylene (BioClear) or in a xylene substitute (BioClear New)
2 exchanges, 2 minutes each
Immediately after clearing apply an appropriate BioMount medium for covering/mounting on the section. If BioClear xylene was used, use one of BioGnost's mounting xylene-based media (BioMount, BioMount High, BioMount M, BioMount DPX, BioMount C, or universal BioMount New). If BioClear New xylene substitute was used, the appropriate covering agent is BioMount New. Cover the section with a VitroGnost cover glass.
P.A.S. Diastase Kit
BioGnost’s P.A.S. Diastase kit is most commonly used for identifying glycogen in liver. Periodic acid enables the molecules containing glycol groups to create aldehydes affected by Schiff’s reagent staining them violet (magenta). Specific stains are created by applying the PAS method on unsubsti-tuted polysaccharides, mucoproteins and glycoproteins, glycolipids and phospholipids. Alpha-amylase enzyme (also known as diastasis) is used for differentiation between glycogen and other PAS-positive structures by dissolving 1→4 glycosidic bonds, causing the glycogen to remain unstained after the PAS reaction. BioGnost’s P.A.S. Diastase kit uses thermostable enzyme which does not require heating to +37°C to be active, but incubat-ing the section at +37°C is preferred in order to achieve better glycogen breakdown. The same tissue section is used as negative control for this reaction, but the sample is not treated using alpha-amylase.
For 100 tests.
Giemsa HP kit
Four-reagent kit for staining Helicobacter pylori in gastroscopic sections according to Lennart. Advantages of this method for detecting H. pylori are sensitive and reproducible results and easy performance.
Alcian Yellow Toluidine Blue kit
Six-reagent kit for staining Helicobacter pylori in gastric tissue sections. This method is one of the most popular non-silver methods for staining of H. pylori, where bacteria are stained blue in contrast to yellow mucins.
Field kit 500ml
Ready to use two-reagent kit for rapid and efficient staining and detection of parasites in haematology samples. Primarily used for staining thin and thick blood smears (dense drop) for purpose of diagnosing blood parasites. Reagents are stored in containers that can be used as staining jars.
Papanicolaou Rapid Staining Kit, for 100 Tests
Ready-to-use eight-reagent kit (in 18 containers that can be used as staining jars) for rapid progressive gynecology and non-gynecology cytological samples. Contains xylene substitute as clearing agent and xylene substitute-based medium for permanent covering of samples.
PAP-100T for 100 tests
TB-Stain Auramine O Kit
Three-reagent kit for staining acid-fast bacteria using fluorescence method. Contains TB Auramine O reagent, double amount of TB Decolorizer Fluorescent and counterstain of TB Permanganate reagent.
4 x 100ml bottles.