The most common and renowned method used for detecting tuberculosis bacteria is staining according to Ziehl-Neelsen. This method uses Carbol Fuchsin as the main dye, acid alcohol as decolourisation medium and Methylene Blue solution as a contrasting dye. BioGnost’s TB-Stain Hot kit contains TB Carbol Fuchsin reagent, two packages of TB Decolorizer and Methylene Blue Loeffler reagent.
TB-Stain Hot Kit
Three-reagent kit for staining acid-fast bacteria. Contains TB Carbol Fuchsin reagent, double amount of TB Decolorizer and Methylene Blue Loeffler’s reagent as counterstain.
4 x 100ml bottles.
Description
PROCEDURE
Using kit for 100 tests | ||
1 | Cover the samples completely with the TB Carbol Fuchsin reagent. Carefully heat the glass slide containing the sample | 5 minutes |
2 | Rinse with tap water until the dye destains. | |
3 | Cover the sample using using TB Decolorizer and let it set for 15-30 seconds (depending on the sample thickness). | 15-30 seconds |
4 | Rinse with tap water. | |
5 | Stain the sample using BioGnost's Methylene Blue Loeffler reagent. | 30 seconds |
6 | Rinse with tap water thoroughly. | |
7 | Dry the section. | |
Microbiology staining procedures are not standardized and they depend on standard operating procedures of individual laboratories and the |
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