The most common and renowned method used for detecting tuberculosis bacteria is staining according to Ziehl-Neelsen. This method uses Carbol Fuchsin as the main dye, acid alcohol as decolourisation medium and Methylene Blue solution as a contrasting dye. BioGnost’s TB-Stain Hot kit contains TB Carbol Fuchsin reagent, two packages of TB Decolorizer and Methylene Blue Loeffler reagent.
TB-Stain Hot Kit
Three-reagent kit for staining acid-fast bacteria. Contains TB Carbol Fuchsin reagent, double amount of TB Decolorizer and Methylene Blue Loeffler’s reagent as counterstain.
4 x 100ml bottles.
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Stain Kits
TB-Stain Fluorescent Kit
Three-reagent kit for fluorescence-microscopic detection of acid-fast bacteria. Contains TB Auramine-Rhodamine reagent, double amount of TB Decolorizer Fluorescent and TB Permanganate reagent as counterstain.
4 x 100ml bottles.
Stain Kits
TB-Stain Auramine O Kit
Three-reagent kit for staining acid-fast bacteria using fluorescence method. Contains TB Auramine O reagent, double amount of TB Decolorizer Fluorescent and counterstain of TB Permanganate reagent.
4 x 100ml bottles.
Stain Kits
TB-Stain Cold Kit
Three-reagent kit for staining acid-fast bacteria according to Kinyoun. Contains TB Carbol Fuchsin reagent, double amount of TB Decolorizer and TB Malachite Green reagent as counterstain.
4 x 100ml bottles.
Stain Kits
BioGram ECO kit
Four-reagent phenol-free kit for the identification of bacteria according to Gram. Kit contains Gram Crystal violet, phenol free reagent, Gram Sodium hydrogencarbon, solution, stabilized Gram Lugol solution, double amount of Gram Decolorizer solution 2 and Gram Safranin solution as counterstain.
2×50 mL+4×100 mL bottles
Stain Kits
BioGram 4 kit
Four-reagent kit for identification of bacteria according to Gram. Kit contains Gram Crystal Violet 1% solution, stabilized Gram Lugol solution, double amount of Gram Decolorizer solution 2 and Gram Safranin solution as counterstain.
5×100 ml bottles
Stain Kits
Elastica-Van Gieson kit
Four-reagent kit for staining elastic fibres and differentiation between elastic tissue, collagen and other types of connective tissue. The rapid method enables a satisfactory result with shorter section staining time.
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P.A.S. Diastase Kit
BioGnost’s P.A.S. Diastase kit is most commonly used for identifying glycogen in liver. Periodic acid enables the molecules containing glycol groups to create aldehydes affected by Schiff’s reagent staining them violet (magenta). Specific stains are created by applying the PAS method on unsubsti-tuted polysaccharides, mucoproteins and glycoproteins, glycolipids and phospholipids. Alpha-amylase enzyme (also known as diastasis) is used for differentiation between glycogen and other PAS-positive structures by dissolving 1→4 glycosidic bonds, causing the glycogen to remain unstained after the PAS reaction. BioGnost’s P.A.S. Diastase kit uses thermostable enzyme which does not require heating to +37°C to be active, but incubat-ing the section at +37°C is preferred in order to achieve better glycogen breakdown. The same tissue section is used as negative control for this reaction, but the sample is not treated using alpha-amylase.
For 100 tests.
Stain Kits
Grocott kit, stabilised
Seven-reagent kit for visualization of fungi and histological argentaffin structures in general (such as basal membranes). Green counterstain provides clear and visually rich contrast to target structures stained black.