TB-Stain Hot Kit

Using kit for 100 tests

1

 Cover the samples completely with the TB Carbol Fuchsin reagent. Carefully heat the glass slide containing the sample
and dye on the bottom side of the slide using the Bunsen burner until evaporation occurs. Keep the slide hot for 5 min. Do
not let the dye boil.

5 minutes

2

Rinse with tap water until the dye destains.

3

Cover the sample using using TB Decolorizer and let it set for 15-30 seconds (depending on the sample thickness).

15-30 seconds

4

 Rinse with tap water.

5

 Stain the sample using BioGnost's Methylene Blue Loeffler reagent.

30 seconds

6

Rinse with tap water thoroughly.

7

Dry the section.

Microbiology staining procedures are not standardized and they depend on standard operating procedures of individual laboratories and the
experience of the personnel conducting the staining procedure. Intensity of staining depends on the period of immersion in the dye. Depending on
personal requests and standard laboratory operating procedures, sample processing and staining can be carried out according to other protocols.