The most common and renowned method used for detecting tuberculosis bacteria is staining according to Ziehl-Neelsen. This method uses Carbol Fuchsin as the main dye, acid alcohol as decolourisation medium and Methylene Blue solution as a contrasting dye. BioGnost’s TB-Stain Hot kit contains TB Carbol Fuchsin reagent, two packages of TB Decolorizer and Methylene Blue Loeffler reagent.
TB-Stain Hot Kit
Three-reagent kit for staining acid-fast bacteria. Contains TB Carbol Fuchsin reagent, double amount of TB Decolorizer and Methylene Blue Loeffler’s reagent as counterstain.
4 x 100ml bottles.
Related products
Stain Kits
TB-Stain Fluorescent Kit
Three-reagent kit for fluorescence-microscopic detection of acid-fast bacteria. Contains TB Auramine-Rhodamine reagent, double amount of TB Decolorizer Fluorescent and TB Permanganate reagent as counterstain.
4 x 100ml bottles.
Stain Kits
BioGram Histo kit
Five-reagent kit for identification of bacteria according to Gram. For differentiation between Gram-positive and Gram-negative bacteria in histology sections.
HE Rapid Staining kit- frozen and paraffin sections
Ready-to-use eight-reagent kit (in 16 containers that can be used as staining jars) for rapid HE staining of frozen and paraffin tissue sections in histopathology. Contains xylene substitute as clearing agent and xylene substitute-based medium for permanent section covering.
For 100 tests.
Stain Kits
TB-Stain ECO Kit
Three-reagent phenol-free kit for staining acid-fast bacteria. Contains TB-Fuchsin reagent, double amount of TB Decolorizer and Methylene Blue Loeffler’s reagent as counterstain.
5 x 100ml bottles.
Stain Kits
P.A.S. Diastase Kit
BioGnost’s P.A.S. Diastase kit is most commonly used for identifying glycogen in liver. Periodic acid enables the molecules containing glycol groups to create aldehydes affected by Schiff’s reagent staining them violet (magenta). Specific stains are created by applying the PAS method on unsubsti-tuted polysaccharides, mucoproteins and glycoproteins, glycolipids and phospholipids. Alpha-amylase enzyme (also known as diastasis) is used for differentiation between glycogen and other PAS-positive structures by dissolving 1→4 glycosidic bonds, causing the glycogen to remain unstained after the PAS reaction. BioGnost’s P.A.S. Diastase kit uses thermostable enzyme which does not require heating to +37°C to be active, but incubat-ing the section at +37°C is preferred in order to achieve better glycogen breakdown. The same tissue section is used as negative control for this reaction, but the sample is not treated using alpha-amylase.
For 100 tests.
Stain Kits
TB-Stain Histo kit
Three-reagent kit for staining acid-fast bacteria (pathogenic mycobacteria) in histology sections, sputum, smears and culture smears according to Ziehl-Neelsen. Heating of the carbol-fuchsin solution is avoided in this protocol hence omitting the release of hazardous phenolic vapours.
Stain Kits
BioGram 4 kit
Four-reagent kit for identification of bacteria according to Gram. Kit contains Gram Crystal Violet 1% solution, stabilized Gram Lugol solution, double amount of Gram Decolorizer solution 2 and Gram Safranin solution as counterstain.
5×100 ml bottles
Stain Kits
Eosin-Nigrosin Vital
Fast detection (one-step detection) of sperm vitality and visualisation of dead and living sperm cells with one reagent. A simple, easy and fast method for semen analysis.