Ready-To-Use kit for cytology gynecology and non-gynecology sections staining according to Papanicolaou enables complete sample processing in just a few minutes after sampling. The staining procedure is simplified and conducted in a few minutes. It contains all the reagents necessary for sample processing – 95% alcohol as fixative, deionized water, Hematoxylin HP, nuclear bluing reagent, OG-6 reagent and EA 50 reagent, as well as 100% alcohol for tissue dehydration and BioClear New (xylene substitute) for section clearing. It also contains mounting medium of very low viscosity and optimal refractive index (BioMount New) in practical packaging. The reagents are placed in practical jars and sections may be directly immersed. They are placed in the order of use in the box, which lowers the possibility of contamination of reagents during staining. The kit contains additional jar of Bluing reagent used after staining 50 sections. The kit is sufficient for staining approximately 100 sections.
Papanicolaou Rapid Staining Kit, for 100 Tests
Ready-to-use eight-reagent kit (in 18 containers that can be used as staining jars) for rapid progressive gynecology and non-gynecology cytological samples. Contains xylene substitute as clearing agent and xylene substitute-based medium for permanent covering of samples.
PAP-100T for 100 tests
Description
PROCEDURE
Using kit for 100 tests | ||
1 | Deparaffinize the section in xylene (BioClear) or in a xylene substitute (BioClear New) | 3 exchanges, 10 minutes each |
2 | Rehydrate using 100% alcohol (Histanol 100) | 2 exchanges, 5 and 3 minutes |
3 | Rehydrate using 95% alcohol (Histanol 95) | 2 minutes |
4 | Rehydrate in distilled (demi) water | 2 minutes |
5 | Add Hematoxylin G1 to the sections (≥5 drops) | 5 minutes |
6 | Rinse under tap water | 4 minutes |
7 | Immerse the sections in freshly prepared Congo Red working solution | 18 minutes |
8 | Rinse under tap water | Until excessive dye is rinsed (a few minutes) |
9 | Dehydrate using 70% alcohol (Histanol 70) | 10 dips |
10 | Dehydrate using 95% alcohol (Histanol 95) | 10 dips |
11 | Dehydrate using 100% alcohol (Histanol 100) | 3 minutes |
12 | Clear the section in xylene (BioClear) or in a xylene substitute (BioClear New) | 2 exchanges, 5 minutes each |
Immediately after clearing apply an appropriate BioMount medium for covering/mounting on the section. If BioClear xylene was used, use one of BioGnost's mounting xylene-based media (BioMount, BioMount High, BioMount M, BioMount DPX, BioMount C, or universal BioMount New). If BioClear New xylene substitute was used, the appropriate covering agent is BioMount New. Cover the section with VitroGnost cover glass. | ||
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