Fouchet-Van Gieson kit is used for simultaneous visualisation of bilirubin and collagen in histological samples. Bilirubin is a yellow-brown pigment created as a result of haemoglobin degradation. Haemoglobin degradation occurs in bone marrow, spleen, and liver. In the case of patients that suffer from hepatitis, bilirubin builds up in the form of thrombus in bile ducts and in the form of granules in hepatocytes and in the cytoplasm of Küpfer cells. The pigment is insoluble in water and in water fixatives. However, in cases of prolonged exposure to formalin fixatives it may turn green. The colour that is created during staining using the Fouchet-Van Gieson kit is due to the strong oxidoreduction of the complex and subsequent conversion to biliverdin (green). The false-positive reaction may be checked using HemoGnost Perls kit – in that case Perls’ reaction will always be negative to bilirubin.
Fouchet-Van Gieson kit
Three-reagent kit for visualisation of bilirubin and collagen according to Kutllick. Bilirubin is a yellow-brown pigment, but changes to green due to oxidation induced by Fouchet reaction. Green bilirubin can easily be detected on yellowish and pink coloured background.
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Additional information
| Size | |
|---|---|
| Brand | |
| Stain pack | |
| Stain Category | Pigments and Mineral Deposits |
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