Grimelius kit is used in histology for visualisation of argyrophilic structures in histology tissue sections. Certain tissues, such as neuroendocrine tumours can bind to silver ions from silver nitrate solution, but not reduce them to the visible form – elementary silver; this is why silver ion reduction during staining is achieved by exposing the section to the reducing hydroquinone solution and sodium sulfite. Excessive unbound silver ions are removed by rinsing the section with the sodium thiosulfate solution.
Five-reagent kit for staining argyrophilic granules. Grimelius kit can be used for the detection of secretory intracytoplasmatic granules specific for carcinoid tumours and for identification of neuroendocrine cells.
TB-Stain Fluorescent Kit
Three-reagent kit for fluorescence-microscopic detection of acid-fast bacteria. Contains TB Auramine-Rhodamine reagent, double amount of TB Decolorizer Fluorescent and TB Permanganate reagent as counterstain.
4 x 100ml bottles.
Alcian Yellow Toluidine Blue kit
Six-reagent kit for staining Helicobacter pylori in gastric tissue sections. This method is one of the most popular non-silver methods for staining of H. pylori, where bacteria are stained blue in contrast to yellow mucins.
BioGram ECO kit
Four-reagent phenol-free kit for the identification of bacteria according to Gram. Kit contains Gram Crystal violet, phenol free reagent, Gram Sodium hydrogencarbon, solution, stabilized Gram Lugol solution, double amount of Gram Decolorizer solution 2 and Gram Safranin solution as counterstain.
2×50 mL+4×100 mL bottles
TB-Stain Auramine O Kit
Three-reagent kit for staining acid-fast bacteria using fluorescence method. Contains TB Auramine O reagent, double amount of TB Decolorizer Fluorescent and counterstain of TB Permanganate reagent.
4 x 100ml bottles.
Giemsa HP kit
Four-reagent kit for staining Helicobacter pylori in gastroscopic sections according to Lennart. Advantages of this method for detecting H. pylori are sensitive and reproducible results and easy performance.
Elastica-Van Gieson kit
Four-reagent kit for staining elastic fibres and differentiation between elastic tissue, collagen and other types of connective tissue. The rapid method enables a satisfactory result with shorter section staining time.
P.A.S. Diastase Kit
BioGnost’s P.A.S. Diastase kit is most commonly used for identifying glycogen in liver. Periodic acid enables the molecules containing glycol groups to create aldehydes affected by Schiff’s reagent staining them violet (magenta). Specific stains are created by applying the PAS method on unsubsti-tuted polysaccharides, mucoproteins and glycoproteins, glycolipids and phospholipids. Alpha-amylase enzyme (also known as diastasis) is used for differentiation between glycogen and other PAS-positive structures by dissolving 1→4 glycosidic bonds, causing the glycogen to remain unstained after the PAS reaction. BioGnost’s P.A.S. Diastase kit uses thermostable enzyme which does not require heating to +37°C to be active, but incubat-ing the section at +37°C is preferred in order to achieve better glycogen breakdown. The same tissue section is used as negative control for this reaction, but the sample is not treated using alpha-amylase.
For 100 tests.