The most common and renowned method used for detecting tuberculosis bacteria is staining according to Ziehl-Neelsen. This method uses Carbol Fuchsin as the main dye, acid alcohol as decolourisation medium and Methylene Blue solution as a contrasting dye. BioGnost’s TB-Stain Hot kit contains TB Carbol Fuchsin reagent, two packages of TB Decolorizer and Methylene Blue Loeffler reagent.
TB-Stain Hot Kit
Three-reagent kit for staining acid-fast bacteria. Contains TB Carbol Fuchsin reagent, double amount of TB Decolorizer and Methylene Blue Loeffler’s reagent as counterstain.
4 x 100ml bottles.
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TB-Stain Auramine O Kit
Three-reagent kit for staining acid-fast bacteria using fluorescence method. Contains TB Auramine O reagent, double amount of TB Decolorizer Fluorescent and counterstain of TB Permanganate reagent.
4 x 100ml bottles.
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P.A.S. Diastase Kit
BioGnost’s P.A.S. Diastase kit is most commonly used for identifying glycogen in liver. Periodic acid enables the molecules containing glycol groups to create aldehydes affected by Schiff’s reagent staining them violet (magenta). Specific stains are created by applying the PAS method on unsubsti-tuted polysaccharides, mucoproteins and glycoproteins, glycolipids and phospholipids. Alpha-amylase enzyme (also known as diastasis) is used for differentiation between glycogen and other PAS-positive structures by dissolving 1→4 glycosidic bonds, causing the glycogen to remain unstained after the PAS reaction. BioGnost’s P.A.S. Diastase kit uses thermostable enzyme which does not require heating to +37°C to be active, but incubat-ing the section at +37°C is preferred in order to achieve better glycogen breakdown. The same tissue section is used as negative control for this reaction, but the sample is not treated using alpha-amylase.
For 100 tests.
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Bio-Diff kit 3 x 500ml
Three-reagent kit that contains fi xative agent, red and blue components for fast and effective staining. Each kit contains buffer tablets for consistent staining results.
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Gomori Trichrome kit
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HE Rapid Staining kit- frozen and paraffin sections
Ready-to-use eight-reagent kit (in 16 containers that can be used as staining jars) for rapid HE staining of frozen and paraffin tissue sections in histopathology. Contains xylene substitute as clearing agent and xylene substitute-based medium for permanent section covering.
For 100 tests.
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H.B.F.P. kit
Three-reagent Hematoxylin-Basic Fuchsin-Picric acid staining kit for detection of cardiac muscle changes after ischemia or myocardial infarction. H.B.F.P. kit is a non-enzymatic histochemical technique for detection of early myocardial ischemia with vivid contrast.