BioGnost’s Sudan Black B kit for haematological staining in cytochemistry is used for staining neutrophil granules in blood smears and bone marrow smears. The result of staining the leukocytes with Sudan Black B dye is similar to the result of myeloperoxidase activity. Cells of lymphoid line will not be stained with Sudan Black B dye, while myeloid and monocytoid cells will demonstrate characteristic positive reaction. This is the reason why Sudan Black B is used in methods of determining myelocytic and myelomonocytic leukaemia.
Sudan Black B kit
Four-reagent kit for staining neutrophil granules in haematological smears (blood or bone marrow films). Used as one of the methods for detecting myelocytic and myelomonocytic leukaemia.
BioGram 4 kit
Four-reagent kit for identification of bacteria according to Gram. Kit contains Gram Crystal Violet 1% solution, stabilized Gram Lugol solution, double amount of Gram Decolorizer solution 2 and Gram Safranin solution as counterstain.
5×100 ml bottles
TB-Stain Auramine O Kit
Three-reagent kit for staining acid-fast bacteria using fluorescence method. Contains TB Auramine O reagent, double amount of TB Decolorizer Fluorescent and counterstain of TB Permanganate reagent.
4 x 100ml bottles.
TB-Stain Fluorescent Kit
Three-reagent kit for fluorescence-microscopic detection of acid-fast bacteria. Contains TB Auramine-Rhodamine reagent, double amount of TB Decolorizer Fluorescent and TB Permanganate reagent as counterstain.
4 x 100ml bottles.
P.A.S. Diastase Kit
BioGnost’s P.A.S. Diastase kit is most commonly used for identifying glycogen in liver. Periodic acid enables the molecules containing glycol groups to create aldehydes affected by Schiff’s reagent staining them violet (magenta). Specific stains are created by applying the PAS method on unsubsti-tuted polysaccharides, mucoproteins and glycoproteins, glycolipids and phospholipids. Alpha-amylase enzyme (also known as diastasis) is used for differentiation between glycogen and other PAS-positive structures by dissolving 1→4 glycosidic bonds, causing the glycogen to remain unstained after the PAS reaction. BioGnost’s P.A.S. Diastase kit uses thermostable enzyme which does not require heating to +37°C to be active, but incubat-ing the section at +37°C is preferred in order to achieve better glycogen breakdown. The same tissue section is used as negative control for this reaction, but the sample is not treated using alpha-amylase.
For 100 tests.