One of the most frequently used chemical methods in histology is P.A.S. staining. The P.A.S. staining is based on the oxidation reaction with the presence of periodic acid and Schiff’s reagent. Periodic acid makes the molecules containing glycol groups create aldehydes affected by Schiff’s reagent that stains them violet (magenta). This method is most commonly used in liver and muscle cells testing. Specific stains are created by applying P.A.S. method on unsubstituted polysaccharides, mucoproteins and glycoproteins, glycolipids and phospholipids. Combined with Alcian Blue, it can detect acid mucosubstances (glycosaminoglycans).
Five-reagent Periodic Acid-Schiff kit for staining aldehydes, muccopolysaccharides, mucoproteins and lymphocytes according to Hotchkiss-McManus. P.A.S. staining may also be used for the demonstration of fungal organisms in tissue sections.
Alcian Yellow Toluidine Blue kit
Six-reagent kit for staining Helicobacter pylori in gastric tissue sections. This method is one of the most popular non-silver methods for staining of H. pylori, where bacteria are stained blue in contrast to yellow mucins.
TB-Stain Fluorescent Kit
Three-reagent kit for fluorescence-microscopic detection of acid-fast bacteria. Contains TB Auramine-Rhodamine reagent, double amount of TB Decolorizer Fluorescent and TB Permanganate reagent as counterstain.
4 x 100ml bottles.
Martius Scarlet Blue (MSB) kit, 6x100ml+1x250ml
Seven-reagent kit used for fibrin visualisation, especially of older clusters. This method is a modification of Masson Trichrome and is ideal for studying connective tissue and vascular pathology.
Grocott kit, stabilised
Seven-reagent kit for visualization of fungi and histological argentaffin structures in general (such as basal membranes). Green counterstain provides clear and visually rich contrast to target structures stained black.
Warthin Starry kit
Five-reagent kit for staining Spirochaeta, Helicobacter pylori, Microsporidia and Legionella pneumophila. The kit contains 12 jars with gelatine that enables both incubation and staining of sections, as well as other reagents that enable precipitation of silver on the bacterial surface. The bacteria are found in the mucus of the surface epithelium, in the apical gastric glands and in the gastric mucosa.