Nuclear Fast Red (Kernechtrot) reagent is primarily used as counterstain solution in certain special kits for staining nuclei using red dye. It most commonly binds with aluminum ions and it is used for staining sperm and spermatids in ejaculate, as well as for identifying PCR tubes in tissue sample embedded in paraffin.
Nuclear Fast Red (Kernechtrot) Reagent
Nuclear Fast Red dye solution for staining nuclei. Counterstain in many special staining kits.
Description
Additional information
Stain Category | Cytology, Histology |
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Size |
PROCEDURE
100ml | |
1 | Shake Eosin-nigrosin vital reagent bottle well |
2 | Mix equal volumes of Eosin-nigrosin vital reagent and ejaculate sample (for example, mix 50 µL of Eosin and nigrosine reagent with |
3 | Mix well |
4 | Let it set for 30 seconds at room temperature |
5 | Prepare a glass slide that will contain a thin smear of the stained sample |
6 | Smear the sample on the side |
Note: smear the sample according to the standardized method used in the laboratory or according to the following instructions:
| |
7 | Dry the sections |
Note: if you wish to keep the sections, apply a suitable BioMount medium on the sample (such as BioMount DPX) and cover it with a glass slide | |
8 | Study the sample under a microscopical magnification factor of 40x or under an immersion lens (100x magnification factor) |
Note: image under immersion lens 100x magnification will very clearly show the difference between stained and non-stained sperm cells | |
9 | Count 200 sperm cells; non-stained are classified as living sperm cells, while those stained red or pink are classified as dead |
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