Masson Fontana kit is used in a specific method for proving argentaffin granules in histological sections, based on the reduction of silver nitrate to elemental silver. Melanin is a pigment usually found in skin, hair, retina, and some parts of the central nervous system. In order to avoid getting false-positive results, BioGnost’s Masson Fontana kit contains reagents for melanin depigmentation. Depigmentation is conducted on a control section before silver impregnation.
Masson Fontana kit
Six-reagent melanin and argentaffin granule staining kit, based on the reduction of silver nitrate to elemental silver. Melanin is a brown-black pigment normally present in the hair, skin, retina, iris and certain parts of CNS. Argentaffin granules are found in carcinoid tumours.
Description
Additional information
| Size | |
|---|---|
| Brand | |
| Stain pack | |
| Stain Category | Pigments and Mineral Deposits |
Downloads
Related products
Stain Kits
Bio-Diff Kit
Three-reagent kit that contains fixative agent, red and blue components for fast and effective staining. Each kit contains buffer tablets for consistent staining results.
3×100 ml bottles
Stain Kits
TB-Stain Quick Kit
Three-reagent kit for rapid staining of acid-fast bacteria using Kinyoun-Gabbett method. Contains TB Carbol Fuchsin reagent and TB Armand reagent as counterstain.
3 x 100ml bottles.
HE Rapid Staining kit- frozen and paraffin sections
Ready-to-use eight-reagent kit (in 16 containers that can be used as staining jars) for rapid HE staining of frozen and paraffin tissue sections in histopathology. Contains xylene substitute as clearing agent and xylene substitute-based medium for permanent section covering.
For 100 tests.
Stain Kits
P.A.S. Diastase Kit
BioGnost’s P.A.S. Diastase kit is most commonly used for identifying glycogen in liver. Periodic acid enables the molecules containing glycol groups to create aldehydes affected by Schiff’s reagent staining them violet (magenta). Specific stains are created by applying the PAS method on unsubsti-tuted polysaccharides, mucoproteins and glycoproteins, glycolipids and phospholipids. Alpha-amylase enzyme (also known as diastasis) is used for differentiation between glycogen and other PAS-positive structures by dissolving 1→4 glycosidic bonds, causing the glycogen to remain unstained after the PAS reaction. BioGnost’s P.A.S. Diastase kit uses thermostable enzyme which does not require heating to +37°C to be active, but incubat-ing the section at +37°C is preferred in order to achieve better glycogen breakdown. The same tissue section is used as negative control for this reaction, but the sample is not treated using alpha-amylase.
For 100 tests.
Stain Kits
Gomori Trichrome kit
Five-reagent kit for staining muscle, collagen fibre and nuclei, contains blue counterstain. The kit can be used to contrast skeletal, cardiac or smooth muscle.
Stain Kits
Elastica-Van Gieson kit
Four-reagent kit for staining elastic fibres and differentiation between elastic tissue, collagen and other types of connective tissue. The rapid method enables a satisfactory result with shorter section staining time.
Martius Scarlet Blue (MSB) kit, 6x100ml+1x250ml
Seven-reagent kit used for fibrin visualisation, especially of older clusters. This method is a modification of Masson Trichrome and is ideal for studying connective tissue and vascular pathology.