BioGnost’s Hematoxylin Instant kit is specially prepared for fast and practical preparation of Hematoxylin Instant that can be used for staining histology and cytology samples. It contains two specially stabilized components that (by dissolving in distilled water) create a formulation of hematoxylin that may be used as a substitute for Harris’ or Gill’s hematoxylin. Hematoxylin Instant provides very precise and clear results of cellular nuclei, and may also be used in progressive and in regressive staining methods. It also does not contain oxidant based on mercury chloride. That makes it environment-friendly.
Haematoxylin Instant Kit, 1L
Two-component kit that contains ready-to-use powders for preparation of hematoxylin reagent for progressive and regressive nuclear staining in histopathology and cytology.
HEMI-K-1L 1 L
Using kit for 100 tests
Deparaffinize the section in xylene (BioClear) or in a xylene substitute (BioClear New)
3 exchanges, 10 minutes each
Rehydrate using 100% alcohol (Histanol 100)
2 exchanges, 5 and 3 minutes
Rehydrate using 95% alcohol (Histanol 95)
Rehydrate in distilled (demi) water
Add Hematoxylin G1 to the sections (≥5 drops)
Rinse under tap water
Immerse the sections in freshly prepared Congo Red working solution
Rinse under tap water
Until excessive dye is rinsed (a few minutes)
Dehydrate using 70% alcohol (Histanol 70)
Dehydrate using 95% alcohol (Histanol 95)
Dehydrate using 100% alcohol (Histanol 100)
Clear the section in xylene (BioClear) or in a xylene substitute (BioClear New)
2 exchanges, 5 minutes each
Immediately after clearing apply an appropriate BioMount medium for covering/mounting on the section. If BioClear xylene was used, use one of BioGnost's mounting xylene-based media (BioMount, BioMount High, BioMount M, BioMount DPX, BioMount C, or universal BioMount New). If BioClear New xylene substitute was used, the appropriate covering agent is BioMount New. Cover the section with VitroGnost cover glass.
Papanicolaou Rapid Staining Kit, for 100 Tests
Ready-to-use eight-reagent kit (in 18 containers that can be used as staining jars) for rapid progressive gynecology and non-gynecology cytological samples. Contains xylene substitute as clearing agent and xylene substitute-based medium for permanent covering of samples.
PAP-100T for 100 tests
Elastica-Van Gieson kit
Four-reagent kit for staining elastic fibres and differentiation between elastic tissue, collagen and other types of connective tissue. The rapid method enables a satisfactory result with shorter section staining time.
P.A.S. Diastase Kit
BioGnost’s P.A.S. Diastase kit is most commonly used for identifying glycogen in liver. Periodic acid enables the molecules containing glycol groups to create aldehydes affected by Schiff’s reagent staining them violet (magenta). Specific stains are created by applying the PAS method on unsubsti-tuted polysaccharides, mucoproteins and glycoproteins, glycolipids and phospholipids. Alpha-amylase enzyme (also known as diastasis) is used for differentiation between glycogen and other PAS-positive structures by dissolving 1→4 glycosidic bonds, causing the glycogen to remain unstained after the PAS reaction. BioGnost’s P.A.S. Diastase kit uses thermostable enzyme which does not require heating to +37°C to be active, but incubat-ing the section at +37°C is preferred in order to achieve better glycogen breakdown. The same tissue section is used as negative control for this reaction, but the sample is not treated using alpha-amylase.
For 100 tests.
TB-Stain Fluorescent Kit
Three-reagent kit for fluorescence-microscopic detection of acid-fast bacteria. Contains TB Auramine-Rhodamine reagent, double amount of TB Decolorizer Fluorescent and TB Permanganate reagent as counterstain.
4 x 100ml bottles.
Six-reagent kit for visualisation of fungi and histological argentaffin structures in general (such as basal membranes). Green counterstain provides clear and visually rich contrast to target structures stained black.
Fast detection (one-step detection) of sperm vitality and visualisation of dead and living sperm cells with one reagent. A simple, easy and fast method for semen analysis.
TB-Stain Auramine O Kit
Three-reagent kit for staining acid-fast bacteria using fluorescence method. Contains TB Auramine O reagent, double amount of TB Decolorizer Fluorescent and counterstain of TB Permanganate reagent.
4 x 100ml bottles.
Gomori Trichrome kit
Five-reagent kit for staining muscle, collagen fibre and nuclei, contains blue counterstain. The kit can be used to contrast skeletal, cardiac or smooth muscle.