BioGnost’s Hematoxylin Instant kit is specially prepared for fast and practical preparation of Hematoxylin Instant that can be used for staining histology and cytology samples. It contains two specially stabilized components that (by dissolving in distilled water) create a formulation of hematoxylin that may be used as a substitute for Harris’ or Gill’s hematoxylin. Hematoxylin Instant provides very precise and clear results of cellular nuclei, and may also be used in progressive and in regressive staining methods. It also does not contain oxidant based on mercury chloride. That makes it environment-friendly.
Haematoxylin Instant Kit, 1L
Two-component kit that contains ready-to-use powders for preparation of hematoxylin reagent for progressive and regressive nuclear staining in histopathology and cytology.
HEMI-K-1L 1 L
Description
PROCEDURE
Using kit for 100 tests | ||
1 | Deparaffinize the section in xylene (BioClear) or in a xylene substitute (BioClear New) | 3 exchanges, 10 minutes each |
2 | Rehydrate using 100% alcohol (Histanol 100) | 2 exchanges, 5 and 3 minutes |
3 | Rehydrate using 95% alcohol (Histanol 95) | 2 minutes |
4 | Rehydrate in distilled (demi) water | 2 minutes |
5 | Add Hematoxylin G1 to the sections (≥5 drops) | 5 minutes |
6 | Rinse under tap water | 4 minutes |
7 | Immerse the sections in freshly prepared Congo Red working solution | 18 minutes |
8 | Rinse under tap water | Until excessive dye is rinsed (a few minutes) |
9 | Dehydrate using 70% alcohol (Histanol 70) | 10 dips |
10 | Dehydrate using 95% alcohol (Histanol 95) | 10 dips |
11 | Dehydrate using 100% alcohol (Histanol 100) | 3 minutes |
12 | Clear the section in xylene (BioClear) or in a xylene substitute (BioClear New) | 2 exchanges, 5 minutes each |
Immediately after clearing apply an appropriate BioMount medium for covering/mounting on the section. If BioClear xylene was used, use one of BioGnost's mounting xylene-based media (BioMount, BioMount High, BioMount M, BioMount DPX, BioMount C, or universal BioMount New). If BioClear New xylene substitute was used, the appropriate covering agent is BioMount New. Cover the section with VitroGnost cover glass. | ||
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