Congo Red Puchtler kit is used for staining amyloids (amorphous clusters) in histology sections. Despite their variable protein composition, amyloid deposits are linked by fibril beta-sheets formation. Congo Red dye binds to fibrils and form a regular pattern of dye molecules during the process. The structural regularity of the dye under polarised light demonstrates double refraction of light by displaying green colouration. High pH level and ionic strength of solutions according to Puchtler achieve higher dye specificity to amyloid.
Congo Red Puchtler kit
Three-reagent kit for staining amyloids, characteristic by its high ionic strength and pH enhancing the specificity of Congo Red dye binding to amyloid clusters. This method developed by Puchtler remains the gold standard for amyloids in tissue sections. Amyloid clusters have the property of double refraction that enables green colouration under polarised light.
Description
Additional information
Size | |
---|---|
Brand | |
Stain pack | |
Stain Category | Carbohydrates |
Related products
Stain Kits
Bio-Diff Kit
Three-reagent kit that contains fixative agent, red and blue components for fast and effective staining. Each kit contains buffer tablets for consistent staining results.
3×100 ml bottles
Stain Kits
Movat kit
Ten-reagent kit for staining collagen, elastic and muscle fibres, mucin and fibrin in tissue section. Movat kit is particularly useful when examining heart and vascular diseases.
Stain Kits
Hematoxalyin Instant Kit
Two-component kit that contains ready-to-use powders for the preparation of hematoxylin reagent for progressive and regressive nuclear staining in histopathology and cytology.
1 Litre bottles
Martius Scarlet Blue (MSB) kit, 6x100ml+1x250ml
Seven-reagent kit used for fibrin visualisation, especially of older clusters. This method is a modification of Masson Trichrome and is ideal for studying connective tissue and vascular pathology.
Stain Kits
TB-Stain Histo kit
Three-reagent kit for staining acid-fast bacteria (pathogenic mycobacteria) in histology sections, sputum, smears and culture smears according to Ziehl-Neelsen. Heating of the carbol-fuchsin solution is avoided in this protocol hence omitting the release of hazardous phenolic vapours.
Stains Reagents And Dyes
Rapid Romanowsky (Diff Quik) Stain Kit 3×0.5L
Stain Kits
P.A.S. Diastase Kit
BioGnost’s P.A.S. Diastase kit is most commonly used for identifying glycogen in liver. Periodic acid enables the molecules containing glycol groups to create aldehydes affected by Schiff’s reagent staining them violet (magenta). Specific stains are created by applying the PAS method on unsubsti-tuted polysaccharides, mucoproteins and glycoproteins, glycolipids and phospholipids. Alpha-amylase enzyme (also known as diastasis) is used for differentiation between glycogen and other PAS-positive structures by dissolving 1→4 glycosidic bonds, causing the glycogen to remain unstained after the PAS reaction. BioGnost’s P.A.S. Diastase kit uses thermostable enzyme which does not require heating to +37°C to be active, but incubat-ing the section at +37°C is preferred in order to achieve better glycogen breakdown. The same tissue section is used as negative control for this reaction, but the sample is not treated using alpha-amylase.
For 100 tests.
Stain Kits
TB-Stain Fluorescent Kit
Three-reagent kit for fluorescence-microscopic detection of acid-fast bacteria. Contains TB Auramine-Rhodamine reagent, double amount of TB Decolorizer Fluorescent and TB Permanganate reagent as counterstain.
4 x 100ml bottles.