Congo Red Puchtler kit is used for staining amyloids (amorphous clusters) in histology sections. Despite their variable protein composition, amyloid deposits are linked by fibril beta-sheets formation. Congo Red dye binds to fibrils and form a regular pattern of dye molecules during the process. The structural regularity of the dye under polarised light demonstrates double refraction of light by displaying green colouration. High pH level and ionic strength of solutions according to Puchtler achieve higher dye specificity to amyloid.
Congo Red Puchtler kit
Three-reagent kit for staining amyloids, characteristic by its high ionic strength and pH enhancing the specificity of Congo Red dye binding to amyloid clusters. This method developed by Puchtler remains the gold standard for amyloids in tissue sections. Amyloid clusters have the property of double refraction that enables green colouration under polarised light.
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